Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Crk2 around the phosphorylation site of tyrosine 221.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q64010
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
Crk2 (Ab-221) antibody detects endogenous levels of total Crk2 protein.
References:
Ogawa S, et al. (1994) Oncogene 9:1669-1678.
Abassi Y.A., et al. (2002) EMBO J. 21:4571-4582.
Feller S.M., et al. (1994) EMBO J. 13:2341-2351.
Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318.
Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.
Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.
Immunohistochemical analysis of paraffin- embedded human lung adenocarcinoma tissue using Crk2 (Ab-221) Antibody (E022070).