Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CHK1 around the phosphorylation site of serine 317.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Rat
SwissProtNo:
O14757
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
CHK1 (Ab-317) antibody detects endogenous levels of total CHK1 protein.
References:
Dephoure N, et al. (2008) Proc Natl Acad Sci U S A 105, 10762-7.
Cruet-Hennequart S, et al. (2008) DNA Repair (Amst) 7, 582-96.
Matsuoka S, et al. (2007) Science 316, 1160-6.
Carlessi L, et al. (2007) Mol Cancer Ther 6, 935-44.
Stiff T, et al. (2006) EMBO J 25, 5775-82.
Immunohistochemical analysis of paraffin- embedded human lung adenocarcinoma tissue using CHK1 (Ab-317) Antibody (E022066).