Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ASK1 around the phosphorylation site of serine 83.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
Q99683
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity Sensitivity:
ASK1 (Ab-83) antibody detects endogenous levels of total ASK1 protein.
References:
Mochizuki T, et al. (2006) Oncogene 25, 3699-707.
Song JJ, Lee YJ (2005) J Biol Chem 280, 26845-55.
Ichijo, H. et al. (1997) Science 275, 90-94.
Wang, X.S. et al. (1996) J. Biol. Chem. 271, 31607-31611.
Matsuzawa, A. and Ichijo, H. (2001) J. Biochem. (Tokyo) 130, 1-8.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using ASK1 (Ab-83) Antibody (E022058).