Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MDM2 around the phosphorylation site of Serine 166 (A-I-SP-E-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen .
Reactivity:
Human
SwissProtNo:
Q00987
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
MDM2 (Ab-166) Antibody detects endogenous levels of total MDM2 protein.
References:
Haupt, Y. et al. (1997) Nature 387, 296-299.
Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.
Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MDM2 (Ab-166) Antibody (E021550).