Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 576/577 (T-Y-YP-K-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen .
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05397
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
FAK (Ab-576/577) Antibody detects endogenous levels of total FAK protein.
References:
Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK (Ab-576/577) Antibody (E021545).