Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-TP-P-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen .
Reactivity:
Human
SwissProtNo:
P24864
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
Cyclin E1 (Ab-395) Antibody detects endogenous levels of total Cyclin E1 protein.
References:
Lauper, N. et al. (1998) Oncogene 17, 2637-2643.
Won, K.A. and Reed, S.I. (1996) EMBO J. 15, 4182-4193.
Ye, X. et al. (2004) J. Biol. Chem. 279, 50110-50119.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin E1 (Ab-395) Antibody (E021541).