Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 88 (P-V-YP-G-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen .
Reactivity:
Human
SwissProtNo:
P49023
Applications:
WB:1:500~1:1000 IF:1:100~1:200
Specificity Sensitivity:
Paxillin (Ab-88) Antibody detects endogenous levels of total Paxillin protein.
References:
Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441
Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.
Immunofluorescence staining of methanol-fixed HeLa
cells using Paxillin (Ab-88) Antibody (E021538).
Peptide + -
Western blot analysis of extracts from HUVEC cell using Paxillin (Ab-88) Antibody (E021538).