Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PLC-γ2 around the phosphorylation site of tyrosine 1217 (F-L-YP-D-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P16885
Applications:
IHC: 1:50~1:100 IF:1:100~1:200
Specificity Sensitivity:
PLC-γ2 (Ab-1217) Antibody detects endogenous levels of total PLC-γ2 protein.
References:
Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Smrcka, A. V. et al. (1991) Science 251, 804-807.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-γ2 (Ab-1217) Antibody (E021524).
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ2 (Ab-1217) Antibody (E021524, Red).