Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human α-catenin around the phosphorylation site of tyrosine 177 (I-Q-YP-K-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P35221
Applications:
WB:1:500~1000 IHC: 1:50~1:100
Specificity Sensitivity:
α-catenin (Ab-177) Antibody detects endogenous levels of totalα-catenin protein.
References:
Hwang, S.G. et al. (2005) J. Biol. Chem. 280, 12758-12765
Drees, F. et al. (2005) Cell 123, 903-915.
Yamada, S. et al. (2005) Cell 123, 889-901.
Kobielak, A. and Fuchs, E. (2004) Nat. Rev. Mol. Cell Biol. 5, 614-625.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using α -catenin (Ab-177) Antibody (E021521).
Western blot analysis of extracts from SW626 cell usingα-catenin (Ab-177) Antibody (E021521).