Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from
Human eIF4B around the phosphorylation site of Serine 422 (T-G-SP-E-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse
SwissProtNo:
P23588
Applications:
IHC: 1:50-1:100
Specificity Sensitivity:
eIF4B (Ab-422) Antibody detects endogenous levels of total eIF4B protein.
References:
Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B (Ab-422) Antibody (E021513).