Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human eIF2a around the phosphorylation site of Serine 49 (L-L-SP-E -L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen .
Reactivity:
Human , Mouse , Rat
SwissProtNo:
P05198
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
eIF2α (Ab-49) Antibody detects endogenous levels of total eIF2a protein.
References:
Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Ab-49) Antibody (E021511).