Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from
human Her3/ErbB3 around the phosphorylation site of Tyr1289 (Q-G-YP-E-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed
by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P21860
Applications:
IF:1:100~1:200
Specificity Sensitivity:
Her3/ErbB3 (Ab-1289) Antibody detects endogenous levels of total Her3/ErbB3 protein.
References:
Yarden, Y. and Sliwkowski, M.X. (2001) Nature Rev. Mol. Cell. Biol. 2, 127-137.
Guy, P.M. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8132-8136.
Songyang, Z. et al. (1993) Cell 72, 767-778.
Kim, H.H. et al. (1994) J. Biol. Chem. 269, 24747-24755.
Immunofluorescence staining of methanol-fixed MCF cells using Her3/ErbB3 (Ab-1289) Antibody (E021509, Red).