Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PI3K P85 alpha/gamma/beta around the phosphorylation site of tyrosine 467/199/464 (L-YP-
E-E-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P27986 Q92569 O00459
Applications:
IHC: 1:50-1:100 WB: 1:500-1:1000
Specificity Sensitivity:
PI3K P85 α/γ/β (Ab-467/199/464) Antibody detects endogenous levels of total PI3K P85
alpha/gamma/beta protein.
References:
Stoyanov, B. et al. (1995) Science 269, 690-3.
Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PI3K P85 α/γ/β (Ab-467/199/464) Antibody (E021508).