• Catalog Number:

  E021319-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human SHP-2 around the phosphorylation site of tyrosine 542 (H-E-YP-T-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q06124

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  SHP-2 (Ab-542) antibody detects endogenous levels of total SHP-2 protein.

• References:

  Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682 Mañes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SHP-2 (Ab-542) antibody (E021319).

Western blot analysis of extracts from NIH/3T3 cells treated with insulin (100nM, 15min), using SHP-2 (Ab-542) antibody (E021319, Lane 1 and 2).