Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P49137
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
MAPKAPK-2 (Ab-334) antibody detects endogenous levels of total MAPKAPK-2 protein.
References:
Rouse, J. et al. (1994) Cell 78, 1027-1037.
Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.
Western blot analysis of extract from HeLa cells treated with UV (20min), using MAPKAPK-2 (Ab-334) antibody (E021308, Lane 1 and 2) and MAPKAPK-2 (Phospho-Thr334) antibody (E011308, Lane 3 and 4).