Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Keratin 18 around the phosphorylation site of serine 33 (A-A-SP-V-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P05783
Applications:
IHC: 1:50-1:100
Specificity Sensitivity:
Keratin 18 (Ab-33) antibody detects endogenous levels of total Keratin 18 protein.
References:
Ramaekers, F.C. and Bosman, F.T. (2004) J. Pathol. 204, 351-354.
Chang, L. and Goldman, R.D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613.
Moll, R. et al. (1982) Cell 31, 11-24.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18 (Ab-33) antibody (E021306).