Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human IGF-1R around the phosphorylation site of tyrosine 1280( S-F-YP-Y-S ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08069
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
IGF-1R (Ab-1280) antibody detects endogenous levels of total IGF-1R protein.
References:
Adams, T.E. et al. (2000) Cell. Mol. Life Sci. 57, 1050-1093
Baserga, R. et al. (2000) Oncogene 19, 5574-5581
Scheidegger, K.J. et al. (2000) J. Biol. Chem. 275, 38921-38928.
O'Connor R, et al. Mol Cell Biol 1997 Jan; 17(1): 427-35
Western blot analysis of extract from 293 cells, using IGF-1R (Ab-1280) antibody (E021302, Lane 1 and 2).