• Catalog Number:

  E021297-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human NFκB-p100/p52 around the phosphorylation site of serine 871 (S-Q-SP-V-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  Q00653

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

• Specificity Sensitivity:

  NFκB-p100/p52 (Ab-871) antibody detects endogenous levels of total NFκB-p100/p52 protein.

• References:

  Shin HM, et al. (2006) EMBO J; 25(1): 129-138. Li Q, et al. (2005) Proc Natl Acad Sci USA; 102(35): 12425-12430. Chen C, et al. (2000) Mol Cell Biol; 20(8): 2687-2695.

Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFκB-p100/p52 (Ab-871) antibody (E021297).

Western blot analysis extract from HT-29 cells treated with TNF-α (20ng/ml, 15min) and Calyculin A (50nM, 15min), using NF-κB p100/p52 (Ab-871) antibody (E021297, Lane 1 and 2).