• Catalog Number:

  E021296-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IkB-ε around the phosphorylation site of serine 22 ( I-E-SP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  O00221

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  IkB-ε (Ab-22) antibody detects endogenous levels of total IkB-ε protein.

• References:

  Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74 Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z.J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-ε (Ab-22) antibody (E021296).

Western blot analysis of extract from 293 cells, untreated or treated with TNF-α (20ng/ml, 15min), using IkB-ε (Ab-22) antibody (E021296, Lane 1 and 2) and IkB-ε (Phospho-Ser22) antibody (E011213, Lane 3 and 4).