• Catalog Number:

  E021290-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E)

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08238

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100 IF: 1:100-200

• Specificity Sensitivity:

  HSP90B (Ab-254) antibody detects endogenous levels of total HSP90B protein.

• References:

  Zhao R, Houry WA.(2007) Adv Exp Med Biol; 594: 27-36. Olsen JV, et al. (2006) Cell; 127(3): 635-648 Ogiso H, et al. (2004) Biochemistry; 43(49): 15510-15519

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSP90B (Ab-254) antibody (E021290).

Western blot analysis of extract from HeLa cells, untreated or treated with UV or Heat shock, using HSP90B (Ab-254) antibody (E021290).