• Catalog Number:

  E021280-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from mouse BIM around the phosphorylation site of serine 65 (P-A-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O43521

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  BIM (Ab-65) antibody detects endogenous levels of total BIM protein.

• References:

  Fukazawa H, et al. (2004) Mol Cancer Ther ; 3(10): 1281-1288 Luciano F, et al. (2003) Oncogene ; 22(43): 6785-6793

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using BIM (Ab-65) antibody (E021280).

Western blot analysis of extract from Jurkat cells untreated or treated with H2O2, using BIM (Ab-65) antibody (E021280).