Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P23443
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p70 S6 Kinase (Ab-411) antibody detects endogenous levels of total p70 S6 Kinase protein.
References:
Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851
Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84.
Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c
Le, X.F, et al. (2003) Oncogene 22: 484–97
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p70 S6 Kinase (phospho-Ser424) antibody (E011284).
Western blot analysis of extracts from 293 cells using p70 S6 Kinase (Ab-424) antibody (E021276).