Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from mouse Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q14247
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
Cortactin (Ab-421) antibody detects endogenous levels of total Cortactin protein.
References:
Luo C, et al. (2006) J Biol Chem ; 281(40):30081-30093
Head JA, et al. (2003) Mol Biol Cell ;14(8):3216-3229
Salomon AR, et al. (2003) PNAS ; 100(2): 443-448
Huang C, et al. (1998) J Biol Chem ; 273(40): 25770-25776.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Cortactin (Ab-421) antibody (E021263).