• Catalog Number:

  E021256-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1110 (P-V-YP-H-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  EGFR (Ab-1110) antibody detects endogenous levels of total EGFR protein.

• References:

  Kaisa Erjala, et al. (2006) Clin. Cancer Res Jul 2006; 12: 4103 - 4111. Judit Anido, et al. (2003) Clin. Cancer Res ; 9: 1274. Julian Andreev, et al. (2001) J. Biol. Chem ; 276: 20130 - 20135. Suil Kim, et al. (2002) Am J Physiol Lung Cell Mol Physiol; 283: 67.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using EGFR (Ab-1110) antibody (E021256).

Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using EGFR (Ab-1110) antibody (E021256, Lane 1 and 2) and EGFR (phospho-Tyr1110) antibody (E011264, Lane 3 and 4).