Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human α-Synuclein around the phosphorylation site of tyrosine 125 ( E-A-YP-E-M ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P37840
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
α-Synuclein (Ab-125) Antibody detects endogenous levels of total α-Synuclein protein.
References:
Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.
Western blot analysis of extract from K562 cells, using α-Synuclein (Ab-125) antibody (E021248, Lane 1 and 2).