Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human RelB around the phosphorylation site of serine 552 (L-L-SP-P-G).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q01201
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
RelB (Ab-552) antibody detects endogenous levels of total RelB protein.
References:
Marienfeld R, et al. (2001) Oncogene. 20 (56): 8142-7.
Charlotte S. Kaetzel1, et al. (2005) Immunological Reviews Volume 206: 83
Elwira Pyz, et al. (2006) J Immunol. 176:7447-55
Western blot analysis of extracts from A431 cells, untreated or treated with EGF (200ng/ml 10min), using RelB (Ab-552) antibody (E021247, Line 1 and 2) and RelB (phospho-Ser552) antibody (E011255, Line 3 and 4).