Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SAPK/JNK around the phosphorylation site of tyrosine 185(T-P-YP-V-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P45984; P53779
Applications:
IHC: 1:50~1:100
Specificity Sensitivity:
SAPK/JNK (Ab-185) antibody detects endogenous levels of total SAPK/JNK protein.
References:
I. Ferrer, et al. (2003) Neuropathology & Applied Neurobiology 29: 23
Zhonghong Guan, et al. (1999) J Biol Chem, Vol. 274: 36200-36206
D.Margriet Ouwens1, et al. (2002)The EMBO Journal 21: 3782–3793,
S Matsuyoshi1, et al. (2006) British Journal of Cancer 94: 532
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Ab-185) antibody (E021242).