Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-YP-V-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P27361
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p44/42 MAP Kinase (Ab-204) antibody detects endogenous levels of total p44/42 MAP Kinase protein.
References:
TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase (Ab-204) antibody (E021238, Line 1 and 2) and p44/42 MAP Kinase (phospho- Tyr204) antibody (E011246, Line 3).