Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from
human eNOS around the phosphorylation site of threonine 495(K-K-TP-F-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29474
Applications:
IF:1:100~1:200
Specificity Sensitivity:
eNOS (Ab-495) Antibody detects endogenous levels of total eNOS protein.
References:
Moreno H Jr.(2008)Breast Cancer Res Treat. 109(1):181-2.
Lorenz M,et al.(2007)FASEB J. May;21(7):1556-64.
Lin HI,et al.(2006)Transplant Proc. Sep;38(7):2203-6.
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-495) Antibody (E021235, Red).