• Catalog Number:

  E021216-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-TP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13541

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  4E-BP1 (Ab-45) antibody detects endogenous levels of total 4E-BP1 protein. This antibody may cross-react with 4E-BP2 and 4E-BP3.

• References:

  Gingras AC, et al. (1998) Genes Dev 12(4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904. Kumar V, et al. (2000) EMBO J 19(5): 1087-1097. Moody CA, et al. (2005) J Virol 79(9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) antibody (E021216).

Western blot analysis of extracts from MDA-MB-435 cells untreated or treated with EGF (200ng/ml, 5min), using 4E-BP1 (Ab-45) antibody (E021216, Lane1 and 2) and 4E-BP1 (phospho-Thr45) antibody (E011223, Lane 3 and 4).