• Catalog Number:

  E021208-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 397 (D-D-YP-A-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Rat

• SwissProtNo:

  Q05397

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  FAK (Ab-397) antibody detects endogenous levels of total FAK protein.

• References:

  von Sengbusch A, et al. (2005) Am J Pathol ; 166(2): 585-596 Fornaro M, et al. (2000) Mol Biol Cell ; 11(7): 2235-2249 Zhang X, et al. (1999) Proc Natl Acad Sci U S A; 96(16): 9021-9026.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FAK (Ab-397) antibody (E021208).

Western blot analysis of extract from HUVEC cells treated with H2O2, using FAK (Ab-397) antibody (E021208, Lane 1 and 2).