• Catalog Number:

  E021206-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  Q00613

• Applications:

  WB: 1:500~1:1000 IHC: 1:50-1:100

• Specificity Sensitivity:

  HSF1 (Ab-307) antibody detects endogenous levels of total HSF1 protein.

• References:

  Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4 Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026 Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSF1 (Ab-307) antibody (E021206).

Western blot analysis of extracts from Hela cells treated with IFN or Heat shock, using HSF1 (Ab-307) antibody (E021206, Lane 1, 2 and 3).