Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human APP around the phosphorylation site of threonine 668 ( A-V-TP-P-E ).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P05067
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
APP (Ab-668) antibody detects endogenous levels of total APP protein.
References:
Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122
Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091
Western blot analysis of extract from mouse brain tissue, using APP (Ab-668) antibody (E021204, Lane 1 and 2) and APP (Phospho-Thr668) antibody (E011190, Lane 3 and 4).