• Catalog Number:

  E021199-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 31(T-P-YP-S-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P49023

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Alter Name:

  PAX-1, PXN, Paired box protein Pax-1, paxillin, paxillin alpha

• Specificity Sensitivity:

  Paxillin (Ab-31) antibody detects endogenous levels of total Paxillin protein.

• References:

  Davidson D, et al. (2001) EMBO J; 20(13): 3414-3426 Kook S, et al. (2000) Mol Biol Cell; 11(3): 929-939 Fleming I, et al. (1999) Proc Natl Acad Sci U S A; 96(3): 1123-1128 Goldberg MB, et al. (2001) Microbiol Mol Biol Rev; 65(4): 595-626 Thomas JT, et al. (1999) Proc Natl Acad Sci U S A; 96(15): 8449-8454

• All Sites:

  Human: Tyr31

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Ab-31) antibody (E021199).

Western blot analysis of extracts from SK-OV3 cells untreated or estradiol-treated (0.1μM, 20min), using Paxillin (Ab-31) antibody (E021199, Line 1 and 2) and Paxillin (phospho-Tyr31) antibody (E011201, Line 3 and 4).