Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B2 around the phosphorylation site of tyrosine 330 (N-I-YP-Y-K).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P52799
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Ephrin-B2 (Ab-330) antibody detects endogenous levels of total Ephrin-B2 protein.
References:
Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al. (2006) Blood; 107(6):2330-2338.
Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101(15):5583-5588.
Western blot analysis of extract from A431 cells untreated or treated with Insulin, using Ephrin-B2 (Ab-330) antibody (E021196).