Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Ephrin-B2 around the phosphorylation site of tyrosine 316 (P-V-YP-I-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P52799
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Ephrin-B2 (Ab-316) antibody detects endogenous levels of total Ephrin-B2 protein.
References:
Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588.
Western blot analysis of extract from HUVEC cells, untreated or treated with Insulin or EGF, using Ephrin-B2 (Ab-316) antibody (E021195).