• Catalog Number:

  E021191-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q13131

• Applications:

  IHC: 1:50-1:100 WB: 1:500-1:1000 IF:1:100~1:200

• Specificity Sensitivity:

  AMPKα1/ AMPKα2 (Ab-174/172) Antibody detects endogenous levels of total AMPK1 protein.

• References:

  Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280(32): 29060-29066 Woods A, et al. (2003) Curr Biol. Nov 11; 13(22): 2004-2008 Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94(2): 631-641 Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97(8): 4023-4028.

Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using AMPKα1/ AMPKα2 (Ab-174/172) Antibody (E021191).

UV + + Peptide + - Western blot analysis of extracts from HT-29 cells, using AMPKα1/ AMPKα2 (Ab-174/172) Antibody (E021191, Lane 1 and 2).