• Catalog Number:

  E021185-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human PKCθ around the phosphorylation site of serine 695 (N-F-SP-F-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04759

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  PKCθ (Ab-695) antibody detects endogenous levels of total PKCθ protein.

• References:

  Xu ZB, et al.(2004) J Biol Chem 279:50401-50409 Thebault S, et al. (2004) Mol Immunol 40: 931-942

Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCθ (Ab-695) antibody (E021185).

Western blot analysis of extract from HT-29 (Lane 1 and 2) and K562 cells (Lane 3) treated with Anisomycin (1mM, 30min), using PKCθ (Ab-695) antibody (E021185).