• Catalog Number:

  E021182-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of threonine 389 (G-F-TP-Y-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000

• Specificity Sensitivity:

  p70 S6 Kinase(Ab-389) antibody detects endogenous levels of total p70 S6 Kinase protein.

• References:

  Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851 Papst PJ, et al. (1998) J Biol Chem. 273(24):15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 c Le, X.F, et al. (2003) Oncogene 22: 484–97

EGF + + Peptide + - Western blot analysis of extract from 293 cells treated with EGF (200ng/ml, 15min), using p70 S6 Kinase (Ab-389) antibody (E021182, Lane 1 and 2).