Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of serine 1176 (T-Q-SP-F-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P29474
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
eNOS (Ab-1176) Antibody detects endogenous levels of total eNOS protein.
References:
Fulton, D. et al. (1999) Nature 399, 597-601.
Harris, M.B. et al. (2001) J. Biol. Chem. 276, 16587-16591.
Thomas, S.R. et al. (2002) J. Biol. Chem. 277, 6017-6024.
Western blot analysis of extract from HUVEC cell, using eNOS (Ab-1176) Antibody (E021170, Lane 1 and 2).