Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human PAK1around the phosphorylation site of threonine 423 (R-S-TP-M-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q13153/Q13177/O75914
Applications:
IHC: 1:50-1:100
Specificity Sensitivity:
PAK1/PAK2/PAK3 (Ab-423/402/421) antibody detects endogenous levels of total PAK1/PAK2/PAK3 protein.
References:
Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 (Ab-423/402/421) antibody (E021169).