Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from mouse Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P19105
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
Myosin Light Chain 2 (Ab-18) antibody detects endogenous levels of total Myosin Light Chain 2 protein.
References:
Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256
Western blot analysis of extract from K562 cells, using Myosin Light Chain 2 (Ab-18) antibody (E021157).