Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P53671
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
LIMK2 (Ab-505) Antibody detects endogenous levels of total LIMK2 protein.
References:
Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.
Western blot analysis of extracts from NIH/3T3 and A2780 cells untreated or treated with UV, using LIMK2 (Ab-505) antibody (E021154).