Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CDC2 around the phosphorylation site of threonine161 (T-Y-TP-H-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06493
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
CDC2 (Ab-161) antibody detects endogenous levels of total CDC2 protein.
References:
Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14
Western blot analysis of the extracts from COLO cells using CDC2 (Ab-161) antibody (E021152).