Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-SP-P-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P46527
Applications:
IHC: 1:50~1:100, WB: 1:500~1:1000
Specificity Sensitivity:
p27Kip1 (Ab-10) antibody detects endogenous levels of total p27Kip1 protein.
References:
Le XF, et al. (2003)J Biol Chem.
Connor MK, et al. (2003)Mol Biol Cell; 14(1): 201-13.
McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28.
Chopra S, et al. (2002)J Biol Chem; 277(36): 32413-6.
Boehm M, et al. (2002)EMBO J; 21(13): 3390-401.