• Catalog Number:

  E021150-1

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-SP-P-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P46527

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100

• Specificity Sensitivity:

  p27Kip1 (Ab-10) antibody detects endogenous levels of total p27Kip1 protein.

• References:

  Le XF, et al. (2003)J Biol Chem. Connor MK, et al. (2003)Mol Biol Cell; 14(1): 201-13. McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28. Chopra S, et al. (2002)J Biol Chem; 277(36): 32413-6. Boehm M, et al. (2002)EMBO J; 21(13): 3390-401.

Peptide - + Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using p27Kip1 (Ab-10) antibody (E021150).

Western blot analysis using p27Kip1 (Ab-10) antibody (E021150): Lane1: The extract from A2780 cells; Lane2: The extract from COLO205 cells; Lane3: The extract from COLO205 cells using E021150 preincubated with synthesized peptide.