• Catalog Number:

  E021145-2

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human cdc25C around the phosphorylation site of serine 216 (S-P-SP-M-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P30307

• Applications:

  IHC: 1:50~1:100 WB: 1:500-1:1000 IF:1:100~1:200

• Specificity Sensitivity:

  cdc25C (Ab-216) antibody detects endogenous levels of total cdc25C protein.

• References:

  Toyoshima-Morimoto F. et al. (2002) EMBO Rep. 3(4): 341-348. Ferguson AM. et al. (2005) Mol Cell Biol. 25(7): 2853-2860. Donzelli M. et al. (2003) EMBO Rep. 4(7): 671-677. Chen F. et al. (2002) Proc Natl Acad Sci U S A. 99(4): 1990-1995.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using cdc25C (Ab-216) antibody (E021145).

Peptide - + Western blot analysis of extract from HT-29 cells, using cdc25C (Ab-216) antibody (E021145, Lane 1 and 2).