Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from
human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P38398
Applications:
IF:1:100~1:200
Specificity Sensitivity:
BRCA1 (Ab-1524) Antibody detects endogenous levels of total BRCA1protein.
References:
Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135
Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13
Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
Immunofluorescence staining of methanol-fixed HeLa cells using BRCA1 (Ab-1524) Antibody (E021139, Red).