Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from mouse p90RSK around the phosphorylation site of threonine 348 (S-R-TP-P-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q15418
Applications:
WB: 1:500~1:1000
Specificity Sensitivity:
p90RSK (Ab-348) antibody detects endogenous levels of total p90RSK protein.
References:
Silverman E, et al. Mol Cell Biol. 2004 Dec; 24(24): 10573-10583.
Andrew D, et al. Biochem J. 2006 February 1; 393(Pt 3): 715–724.
Itoh S, et al. J Biol Chem 2005 Jun 24; 280(25): 24135-24142
Western blot analysis of extract from HeLa cells, untreated or treated with PMA (200nM, 30min), using p90RSK (Ab-348) antibody (E021135, Lane 1 and 2) and p90RSK (phospho-Thr348) antibody (E011105, Lane 3 and 4).