Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of serine 80 (T-H-SP-I-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P45985
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
Specificity Sensitivity:
SEK1/MKK4 (Ab-80) antibody detects endogenous levels of total SEK1/MKK4 protein.
References:
Park HS, et al. (2002) J Biol Chem; 277(4): 2573-8.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue, using SEK1/MKK4 (Ab-80) antibody (E021132).
Peptide - +
Western blot analysis of extracts from NIH/3T3 cells using SEK1/MKK4 (Ab-80) antibody (E021132).